IRF4 antibody (AA 393-407)

Catalog No. ABIN6391353

This anti-IRF4 antibody is a Goat Polyclonal antibody detecting IRF4 in WB, ELISA, IHC and FACS. Suitable for Human and Rat.

Quick Overview for IRF4 antibody (AA 393-407) (ABIN6391353)

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IRF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-IRF4 Antibody

Binding Specificity: AA 393-407

Purpose: IRF4 (aa393-407)

Sequence: DPQRQRKLIT AHVEP

Specificity: This antibody is expected to recognize both reported isoforms (

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Spleen and approx 52 kDa in Rat Spleen lysates (calculated MW of 51.8 kDa according to Human NP_002451.2 and 51.6 kDa according to Human NP_001182215.1 and Rat NP_001099578.1). Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IRF4

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Alternative Name: IRF4

Background: IRF4, interferon regulatory factor 4, LSIRF, MUM1, multiple myeloma oncogene 1

Gene ID: 3662, 16364, 291100

NCBI Accession: NP_002451, NP_001182215

Pathways: Chromatin Binding